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Image Search Results
Journal: Journal of immunology (Baltimore, Md. : 1950)
Article Title: Staphylococcus aureus LukGH promotes formation of neutrophil extracellular traps
doi: 10.4049/jimmunol.1301821
Figure Lengend Snippet: (A) Pore formation. PMNs (1×106) were incubated with 0.1–5.0 nM LukGH or 5.0 nM heat-inactivated (h.i.) LukGH for 30 min, and plasma membrane permeability was evaluated using an EtBr uptake assay. Alternatively, LukGH was incubated with αLukH or IgG isotype control antibody at the indicated concentrations for 15 min prior to combining with PMNs. (B) PMN lysis. PMNs (1×106) were incubated with LukGH, h.i. LukGH, and/or antibody as described in panel A and lysis was determined by release of LDH as described in Methods. *P < 0.05 for the indicated comparisons using a one-way ANOVA and Tukey’s post-test.
Article Snippet: To verify that PMN membrane permeability and lysis were caused by LukGH, we inhibited cytolysis with rabbit polyclonal antibody specific for the
Techniques: Incubation, Clinical Proteomics, Membrane, Permeability, Control, Lysis